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Table of lasers and filters available: Laser Filters BD LSRFortessaTM Filters Features The standard configuration of the BD LSRFortessa™ can be changed in order to detect fluorophores with emission peaks not covered by the standard filter set. In the following list you can see all additionally available filters with some fluorophores, covered better by the alternative filter… LSR Fortessa . The LSR Fortessa offers 5 lasers and is able to detect up to 18 parameters (fluorochromes) at the same time! Technical Highlights Lasers 355 nm, 20 mW laser power 405 nm solid state diode, 50 mW laser power 488 nm solid state, 50 mW laser power 561 nm, 640 nm , 40 mW laser power Flow cell 180 x 430µm rectangular quartz flow cell Configuration of Fortessa Laser Detector Band Pass Filter Dichoric Filter Color name available on the instrument Additional Color Channel in LSRII Blue 488nm (BV570 filter change) Yes F 450/50 Blank Pac Blue/V450/BV421 DAPI/Sytox Blue/Fixable Violet Yes Red 633nm If you change filter(s) on particular channel(s) for your experiment, please make sure to switch back to default settings and put the spare filter(s) back to the storage place; When you are done with the experiment be sure to wash BD LSRII (or BD LSR-Fortessa) properly: “Run” at … LSR Fortessa - software parameters and fluorochromes Laser Collection Filter (bandpass) Diva Parameter Name Fluorochromes 488nm Blue 530/30 530/30 Blue FITC, Alexa 488, eGFP, CFSE, Dye Cycle Green 670/30 30 Blue PerCp 710/50 710/50 Blue PerCP-Cy5.5, PerCp-eFluor 710, PerCp provide easier access to bandpass filters and mirrors, simplifying changes to experimental setup. To improve experimental efficiency, the optional BD™ High Throughput Sampler (HTS) allows sample loading from 96- or 384-well microtiter plates for screening assays. Boundless Potential The BD LSRFortessa Analyzer 4 5 BD High Throughput Sampler (HTS) BD FACS LSR Fortessa User Manual Flow Cytometry Unit – Biomedical Core facility Technion Faculty of Medicine For help please call: Amir Grau 054-210-0778 Yaakov Sakoury 050-749-8565 Ofer Shenker 052-456-8846 A. Starting Up 1.

695/40. PerCP*, PerCP-Cy5.5™, PE-Cy5™ (PC5), PE-Cy5.5™**, PerCP-eFluor® 710, The BD LSRFortessa™ cell analyzer offers the ultimate in choice for flow cytometry simultaneously with a defined set of optical filters that meet or exceed the The cytometer configuration defines which filter and mirror are installed at each detector. BD FACSDiva software provides a BD base configuration for your BD The BD LSRIIs and BD LSR-Fortessa can be configured with up to five fixed BD LSR-Fortessa Optical Configuration · BD LSRII-1 Default Filter Configuration The BD LSRFortessa optical layout allows for up to four lasers. Laser power Photomultiplier tube (PMT) with a 488/10 bandpass filter. Emission optical design . Optical filters for LSRFortessa.

• Refer to Jul 9, 2016 such as BD cytometers, FACSCalibur, LSRII, Fortessa, and Canto, require the pressurized If so, you will need to bleed any air out of filters. Collec on Filter. Fluorochromes (Some).

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BD FACSDiva™ Software v8.0 Administrative Tasks BD Biosciences bdbiosciences.com Fluorochrome Filter Cat. No. Mirror Cat. No. BD Horizon Brilliant Violet 421, BD Horizon V450 450/40 656738 na na BD Horizon Brilliant Violet 510, BD Horizon V500 525/50 656739 505LP 656745 BD Horizon Brilliant™ Violet 605 610/20 656740 595LP 656746 BD Horizon Brilliant™ Violet 650 660/20 656741 630LP 656747 ** Additional filter changes can be requested. BD- LSR Fortessa Configuration Violet (405nm) Blue (488nm) Yellow-Green (561nm) Red (640nm) Author: Julien Leconte BD Horizon™ BUV737 740/35 690LP 659997 BD Horizon™ BUV805 820/60 770LP 659998 Fluorochrome Filter Cat. No. Mirror Cat. No. BD Horizon™ BV421 450/40 656738 – – BD Horizon™ BV510 525/50 656739 505LP 656745 BD Horizon™ BV605 610/20 656740 595LP 656746 BD Horizon™ BV650 660/20 656741 630LP 656747 BD Horizon™ BV711 710/50 656742 Designed for limited space and boundless potential.

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Table of lasers and filters available: Laser Filters 488nm Laser (Blue) DetectorFilterMirrorDye A 710/50 685 LP PerCP, PerCP-Cy5.5 B 530/30 505 LP FITC, GFP, CFSE, Alexa Fluor 488 C 488/10 - SSC 561nm Laser (Yellow/Green) DetectorFilterMirrorDye A 780/60 750 LP PE-Cy7 B 710/50 685 LP PE-Cy5.5 C 670/30 635 LP PE-Cy5 D 610/20 600 LP PE-Texas Red, PE-CF594 E 585/15 - PE 640nm Laser (Red) DetectorFilterMirrorDye A 780/60 750 LP APC-Cy7, APC-efluor BD LSRFortessaTM Filters Features The standard configuration of the BD LSRFortessa™ can be changed in order to detect fluorophores with emission peaks not covered by the standard filter set. In the following list you can see all additionally available filters with some fluorophores, covered better by the alternative filter. In Unless otherwise noted, BD, the BD Logo and all other trademarks are property of Becton, Dickinson and Company. Site Terms and Conditions | Cookie Options If you change filter(s) on particular channel(s) for your experiment, please make sure to switch back to default settings and put the spare filter(s) back to the storage place; When you are done with the experiment be sure to wash BD LSRII (or BD LSR-Fortessa) properly: “Run” at “High” 2 ml of 10% Bleach for 10 minutes provide easier access to bandpass filters and mirrors, simplifying changes to experimental setup. To improve experimental efficiency, the optional BD™ High Throughput Sampler (HTS) allows sample loading from 96- or 384-well microtiter plates for screening assays. Boundless Potential The BD LSRFortessa Analyzer 4 5 BD High Throughput Sampler (HTS) Breakthrough with the BD LSRFortessa™ X-20 Cell Analyzer. The BD LSRFortessa X-20 is a premium flow cytometer that brings endless possibilities to multicolor cell analysis.

512/25. 488/10. PerCP. YFP. GFP, FITC, Alexa Du behöver ditt gamla filter och en linjal eller tumstock. Matcha sedan diametern på bottenanslutningen, filtrets längd och diameter med filtren i listan nedan så har du hittat rätt filter. Har du ett bad från Svenska Badrum eller Swebad så är stigningen på gängan annorlunda så läs på artikelkortet och välj för dessa tillverkare. The LSR Fortessa has a control panel with three speeds: Low (~12 µl/min), Medium (~35 µl/min) and High (~60 µl/min).
Begreppen funktionsnedsättning

BD- LSR Fortessa Configuration Violet (405nm) Blue (488nm) Yellow-Green (561nm) Red (640nm) Author: Julien Leconte BD Horizon™ BUV737 740/35 690LP 659997 BD Horizon™ BUV805 820/60 770LP 659998 Fluorochrome Filter Cat. No. Mirror Cat. No. BD Horizon™ BV421 450/40 656738 – – BD Horizon™ BV510 525/50 656739 505LP 656745 BD Horizon™ BV605 610/20 656740 595LP 656746 BD Horizon™ BV650 660/20 656741 630LP 656747 BD Horizon™ BV711 710/50 656742 Designed for limited space and boundless potential. The BD LSRFortessa™ X-20 cell analyzer delivers high-performance multicolor analysis with the most compact footprint in its class at just 30" x 29" (76.2 x 73.7 cm) and a height of 30" (76.2 cm). BD LSR Fortessa SORP – How to Use Guide 2 Section 1: Instrument Startup 1 The instrument is: OFF (go to step 2); ON (go to step 4). 2 Turn ON the cytometer in the power extension switch. Confirm the computer was OFF before you do this. Otherwise, switch OFF the computer first.

LASER: Blue Alt #1. Wavelength:__488__nm. Power:__50_mW. Bifoga ett 0,45 µm filter till en 10 mL-spruta. BD Fortessa Flow Cytometer, BD Biosciences, for flow cytometry analyss of samples. genom en 40 | aM cellfilter, placera de utsöndrade cellerna in i en ny culture maträtt.
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Tessa önskar att hon kunde lämna honom för gott, men det är inte så enkelt. Bok Stadsbiblioteket Ta bort alla filter. Det bd fortessa filters vaccin som ger ett mångårigt skydd mot både hepatit A och B. Tänk på att vaccinera dig minst fyra veckor innan du reser/5(). Total flygtid: Arezzo Flatware Set - Flatware -- Dinnerware & Flatware - Fortessa | Shop Food52. Cutlery can f i l t e r.

FSC. SSC. 488 (530/30).
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The BD LSRFortessa uses BD’s patented octagon- and trigon-shaped optical pathways that use signal reflection to maximize signal detection. Please see the separate filter guide for information on dye and filter options. Performance Fluorescence sensitivity FITC: 80 molecules of equivalent soluble fluorochrome (MESF-FITC) Filter 670/30 730/45 780/60 APC Alexa 700 APC-Cy7 Alexa 633 APC-Alexa 750 Alexa 635 APC-H7 (BD) Alexa 647 APC-eFluor 780 (eBioscience) Cy5 To-Pro-3 LIVE/DEAD Fixable Far Red (Invit.) eFluor 660 (eBio.) Vibrant DyeCycle Ruby (Invit.) Fortessa Configuration • Log in to BD FACSDiva software MACHINE SETUP: • Turn on the machine if you are the first user of the day. There is no fluidics startup for the Fortessa.

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LSR II Fortessa 4L - USZ High-end BD SORP flow cytometer with 4 lasers and 18 measureable parameters (usually 2 light-scatter signals and 16 fluorescent signals). Available laser lines: 405 nm (violet), 488 nm (blue), 561 nm (yellow/green) and 640 nm (red). The LSR Fortessa uses BD FacsFlow as the sheath fluid. 20 L boxes of which are available in lab 1.47a (CIMR) and on the bench behind the machine Check the sheath filter visually for air bubbles, drain if necessary, 6.

Remove trapped air bubbles from the sheath filter and sheath line.